Products specifications
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Antigen
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Nucleolin (NCL)
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Reactivity
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Human
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Host
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Rabbit
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NCBI Accession
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RPC242Hu03
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Concentration
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200ug/ml
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Conjugate
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No Conjugate
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Immunogen
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Recombinant NCL (Lys388~Gln569) expressed in E.coli.
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Immunoglobulin Type
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IgG
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Purification
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Affinity Chromatography
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Specificity
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The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.
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Application
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WB, ICC, IHC-P, IHC-F, ELISA
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Amino Acid Sequence
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MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-KER DARTLLAKNL PYKVTQDELK EVFEDAAEIR LVSKDGKSKG IAYIEFKTEA DAEKTFEEKQ GTEIDGRSIS LYYTGEKGQN QDYRGGKNST WSGESKTLVL SNLSYSATEE TLQEVFEKAT FIKVPQNQNG KSKGYAFIEF ASFEDAKEAL NSCNKREIEG RAIRLELQGP RGSPNARSQ
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Shipping Conditions
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2 °C - 8 °C
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Storage Temperature
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Store at 4 °C for frequent use. Store at -20 °C to -80 °C for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Handling Instruction
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Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Notes
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All antibodies are custom made on-demand. Please allow 5-7 working days for production and 2-3 days for shipment.
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Legal Information
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Marketed under license from USCN Life Science Inc.
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Optimal working dilutions must be determined by end user.
| Western blotting | 1:100-400 |
| Immunocytochemistry in formalin fixed cells | 1:100-500 |
| Immunohistochemistry in formalin fixed frozen section | 1:100-500 |
| Immunohistochemistry in paraffin section | 1:50-200 |
| Enzyme-linked Immunosorbent Assay | 1:100-200 |