Products specifications
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Antigen
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Vimentin (VIM)
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Reactivity
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Human
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Host
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Rabbit
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NCBI Accession
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RPB040Hu01
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Concentration
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200ug/ml
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Conjugate
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No Conjugate
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Immunogen
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Recombinant VIM (Ser2~Glu466) expressed in E.coli.
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Immunoglobulin Type
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IgG
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Purification
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Affinity Chromatography
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Specificity
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The antibody is a rabbit polyclonal antibody raised against VIM. It has been selected for its ability to recognize VIM in immunohistochemical staining and western blotting.
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Application
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WB, ICC, IHC-P, IHC-F, ELISA
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Amino Acid Sequence
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MGHHHHHHSGSEF-STRSVSSSS YRRMFGGPGT ASRPSSSRSY VTTSTRTYSL GSALRPSTSR SLYASSPGGV YATRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE NTLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHEE EIQELQAQIQ EQHVQIDVDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE AANRNNDALR QAKQESTEYR RQVQSLTCEV DALKGTNESL ERQMREMEEN FAVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR DGQVINETSQ HHDDLE
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Shipping Conditions
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2 °C - 8 °C
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Storage Temperature
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Store at 4 °C for frequent use. Store at -20 °C to -80 °C for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Handling Instruction
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Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Notes
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All antibodies are custom made on-demand. Please allow 5-7 working days for production and 2-3 days for shipment.
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Legal Information
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Marketed under license from USCN Life Science Inc.
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Optimal working dilutions must be determined by end user.
| Western blotting | 1:100-400 |
| Immunocytochemistry in formalin fixed cells | 1:100-500 |
| Immunohistochemistry in formalin fixed frozen section | 1:100-500 |
| Immunohistochemistry in paraffin section | 1:50-200 |
| Enzyme-linked Immunosorbent Assay | 1:100-200 |