816449

ELISA Kit for Vitamin B6 (VB6) General

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Products specifications
Species General
Format 96T
Sample Type Serum, plasma and other biological fluids.
Assay Length 2.5 h
Detection Range 3.7-300ng/mL. The standard curve concentrations used for the ELISA’s were 300ng/mL, 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL
Sensitivity 1.43ng/mL
Specificity This assay has high sensitivity and excellent specificity for detection of Vitamin B6 (VB6). No significant cross-reactivity or interference between Vitamin B6 (VB6) and analogues was observed.
Intra-Assay Precision <10%
Inter-Assay Precision <12%
Storage Temperature -20 °C
Shipping Conditions 2-8 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Vitamin B6 (VB6) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Vitamin B6 (VB6) and unlabeled Vitamin B6 (VB6) (Standards or samples) with the pre-coated antibody specific for Vitamin B6 (VB6). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.
ReagentsQuantity
Pre-coated, ready to use 96-well strip plate1
Standard2
Detection Reagent A1×120µL
Detection Reagent B1×120µL
TMB Substrate1×9mL
Wash Buffer (30 × concentrate)1×20mL
Plate sealer for 96 wells4
Standard Diluent1×20mL
Assay Diluent A1×12mL
Assay Diluent B1×12mL
Stop Solution1×6mL
Instruction manual1

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B6 (VB6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-105%78-93%90-99%84-98%
EDTA plasma(n=5)90-104%81-97%97-104%86-104%
heparin plasma(n=5)80-95%86-105%81-104%93-101%

Matrices listed below were spiked with certain level of recombinant Vitamin B6 (VB6) and the recovery rates were calculated by comparing the measured value to the expected amount of Vitamin B6 (VB6) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)81-8884
EDTA plasma(n=5)83-10292
heparin plasma(n=5)99-105102
Products specifications
Species General
Format 96T
Sample Type Serum, plasma and other biological fluids.
Assay Length 2.5 h
Detection Range 3.7-300ng/mL. The standard curve concentrations used for the ELISA’s were 300ng/mL, 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL
Sensitivity 1.43ng/mL
Specificity This assay has high sensitivity and excellent specificity for detection of Vitamin B6 (VB6). No significant cross-reactivity or interference between Vitamin B6 (VB6) and analogues was observed.
Intra-Assay Precision <10%
Inter-Assay Precision <12%
Storage Temperature -20 °C
Shipping Conditions 2-8 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Vitamin B6 (VB6) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Vitamin B6 (VB6) and unlabeled Vitamin B6 (VB6) (Standards or samples) with the pre-coated antibody specific for Vitamin B6 (VB6). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.