810224

ELISA Kit for Albumin (ALB) Rat

In Stock
*
Please Email support@aspirachemical.com to place your order.

In Stock
Products specifications
Species Rat
Format 96T
Sample Type Serum, plasma and other biological fluids
Assay Length 2.5 h
Detection Range 2.47-200ug/mL. The standard curve concentrations used for the ELISA’s were 200ug/mL, 66.67ug/mL, 22.22ug/mL, 7.41ug/mL, 2.47ug/mL
Sensitivity 0.92ug/mL
Specificity This assay has high sensitivity and excellent specificity for detection of Albumin (ALB). No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
Intra-Assay Precision <10%
Inter-Assay Precision <12%
Storage Temperature -20 °C
Shipping Conditions 2-8 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.
ReagentsQuantity
Pre-coated, ready to use 96-well strip plate1
Standard2
Detection Reagent A1×120µL
TMB Substrate1×9mL
Wash Buffer (30 × concentrate)1×20mL
Plate sealer for 96 wells4
Standard Diluent1×20mL
Assay Diluent A1×12mL
Stop Solution1×6mL
Instruction manual1

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)84-96%92-104%85-102%92-99%
EDTA plasma(n=5)82-105%97-105%94-102%97-105%
heparin plasma(n=5)90-99%92-101%86-96%83-94%

Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-9584
EDTA plasma(n=5)92-10196
heparin plasma(n=5)90-9894
Products specifications
Species Rat
Format 96T
Sample Type Serum, plasma and other biological fluids
Assay Length 2.5 h
Detection Range 2.47-200ug/mL. The standard curve concentrations used for the ELISA’s were 200ug/mL, 66.67ug/mL, 22.22ug/mL, 7.41ug/mL, 2.47ug/mL
Sensitivity 0.92ug/mL
Specificity This assay has high sensitivity and excellent specificity for detection of Albumin (ALB). No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
Intra-Assay Precision <10%
Inter-Assay Precision <12%
Storage Temperature -20 °C
Shipping Conditions 2-8 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.