817310

CLIA Kit for Glial Fibrillary Acidic Protein (GFAP) Rat

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Products specifications
Species Rat
Assay Length 4.5 h
Sample Type Serum, plasma and other biological fluids.
Format 96T
Detection Range 4.12-3000pg/mL The standard curve concentrations used for the CLIA’s were 3000pg/mL, 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL, 4.12pg/mL
Sensitivity 1.7pg/mL
Intra-Assay Precision <10%
Inter-Assay Precision <12%
Specificity This assay has high sensitivity and excellent specificity for detection of Glial Fibrillary Acidic Protein (GFAP). No significant cross-reactivity or interference between Glial Fibrillary Acidic Protein (GFAP) and analogues was observed.
Shipping Conditions 2-8 °C
Storage Temperature -20 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Test Principle The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glial Fibrillary Acidic Protein (GFAP) level in the sample or standard.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.
ReagentsQuantity
Pre-coated, ready to use 96-well strip plate1
Standard2
Detection Reagent A1×120µL
Detection Reagent B1×120µL
Substrate A1×10mL
Wash Buffer (30 × concentrate)1×20mL
Plate sealer for 96 wells4
Standard Diluent1×20mL
Assay Diluent A1×12mL
Assay Diluent B1×12mL
Substrate B1×2mL
Instruction manual1

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glial Fibrillary Acidic Protein (GFAP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)91-99%96-104%95-103%97-104%
EDTA plasma(n=5)87-94%89-96%93-101%83-94%
heparin plasma(n=5)81-94%80-99%86-102%86-95%

Matrices listed below were spiked with certain level of recombinant Glial Fibrillary Acidic Protein (GFAP) and the recovery rates were calculated by comparing the measured value to the expected amount of Glial Fibrillary Acidic Protein (GFAP) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)84-9187
EDTA plasma(n=5)81-9185
heparin plasma(n=5)94-103101
Products specifications
Species Rat
Assay Length 4.5 h
Sample Type Serum, plasma and other biological fluids.
Format 96T
Detection Range 4.12-3000pg/mL The standard curve concentrations used for the CLIA’s were 3000pg/mL, 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL, 4.12pg/mL
Sensitivity 1.7pg/mL
Intra-Assay Precision <10%
Inter-Assay Precision <12%
Specificity This assay has high sensitivity and excellent specificity for detection of Glial Fibrillary Acidic Protein (GFAP). No significant cross-reactivity or interference between Glial Fibrillary Acidic Protein (GFAP) and analogues was observed.
Shipping Conditions 2-8 °C
Storage Temperature -20 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Test Principle The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glial Fibrillary Acidic Protein (GFAP) level in the sample or standard.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.