817190

CLIA Kit for Allograft Inflammatory Factor 1 (AIF1) Rat

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Products specifications
Species Rat
Assay Length 4.5 h
Sample Type Tissue homogenates, cell lysates and other biological fluids
Format 96T
Detection Range 2.74-2000pg/mL The standard curve concentrations used for the CLIA’s were 2000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, 8.23pg/mL, 2.74pg/mL
Sensitivity 1.18pg/mL
Inter-Assay Precision <12%
Intra-Assay Precision <10%
Specificity This assay has high sensitivity and excellent specificity for detection of Allograft Inflammatory Factor 1 (AIF1). No significant cross-reactivity or interference between Allograft Inflammatory Factor 1 (AIF1) and analogues was observed.
Shipping Conditions 2-8 °C
Storage Temperature -20 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Test Principle The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Allograft Inflammatory Factor 1 (AIF1) level in the sample or standard.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.
ReagentsQuantity
Pre-coated, ready to use 96-well strip plate1
Standard2
Detection Reagent A1×120µL
Detection Reagent B1×120µL
Substrate A1×10mL
Wash Buffer (30 × concentrate)1×20mL
Plate sealer for 96 wells4
Standard Diluent1×20mL
Assay Diluent A1×12mL
Assay Diluent B1×12mL
Substrate B1×2mL
Instruction manual1

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Allograft Inflammatory Factor 1 (AIF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-93%79-90%87-99%98-105%
EDTA plasma(n=5)78-99%95-105%84-92%87-104%
heparin plasma(n=5)84-103%94-101%84-104%93-101%

Matrices listed below were spiked with certain level of recombinant Allograft Inflammatory Factor 1 (AIF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Allograft Inflammatory Factor 1 (AIF1) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-8981
EDTA plasma(n=5)80-9287
heparin plasma(n=5)87-9892
Products specifications
Species Rat
Assay Length 4.5 h
Sample Type Tissue homogenates, cell lysates and other biological fluids
Format 96T
Detection Range 2.74-2000pg/mL The standard curve concentrations used for the CLIA’s were 2000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, 8.23pg/mL, 2.74pg/mL
Sensitivity 1.18pg/mL
Inter-Assay Precision <12%
Intra-Assay Precision <10%
Specificity This assay has high sensitivity and excellent specificity for detection of Allograft Inflammatory Factor 1 (AIF1). No significant cross-reactivity or interference between Allograft Inflammatory Factor 1 (AIF1) and analogues was observed.
Shipping Conditions 2-8 °C
Storage Temperature -20 °C
Handling Instruction The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
Test Principle The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Allograft Inflammatory Factor 1 (AIF1) level in the sample or standard.
Notes All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Legal Information Marketed under license from USCN Life Science Inc.